In addition to access to various core facilities in the University of Arizona main campus and the UA Health Sciences campus (north of Speedway), the Division of Translational and Regenerative Medicine has developed three divisional core facilities for faculty and students in the UA Department of Medicine to use in their research projects.
The Divisional core facilities include a:
- Pre-clinical Experimental Animal Core in which rodents (mice and rats) can be used for pulmonary hemodynamic measurement, and pharmacological experiments;
- Hypoxic and Hyperoxic Chamber Core in which small animals (e.g., rats and mice) can be placed in normobaric hypoxic chambers for acute and chronic hypoxic experiments; and
- Human Tissue and Specimen BioBank in which human lung and heart tissues, isolated pulmonary arterial smooth muscle and endothelial cells are stored for immunohitochemical and immunocytochemical experiments, as well as for cell and molecular biological studies.
Pre-Clinical Experiment and Small Animal Core
The Core has two locations: one is in the animal facility in the BIO5 Institute building; the other is located in the AHSC building. The BIO5 animal room is designed for experiments using mice, while the AHSC animal room is designed for experiments using rats. Both animal rooms are equipped with a Millar system and necessary recording device and softways. xperiments, as well as for cell and molecular biological studies.
Hypoxic and Hyperoxic Chamber Core
There are eight normoxic hypoxic chambers in the BIO5 animal room for hypoxic experiments, especially for chronically exposing mice to normobaric hypoxia (10% O2) to develop pulmonary hypertension and lung vascular functional and structural changes.
Human Tissue and Specimen Biobank
There are a total of five freezers in the BioBank for storing blood samples, explanted lung and heart tissues from patients. In addition, there are primary cultured and subcultured pulmonary arterial smooth muscle cells (PASMC) and porcine aortic endothelial cells (PAEC) that are stored in the liquid nitrogen tank and -80°C freezers.
The BioBank works closely with the University of Arizona Health Sciences (UAHS) Biorepository and other biological specimen banking initiatives at UAHS to manage samples and associated pertinent clinical data to facilitate translational research and help investigators accomplish their research aims. The UAHS Biorepository serves as the “biorepository core” for all of the UAHS colleges.
Our Divisional Cores are currently shared by all primary faculty members in the Division of Translational and Regenerative Medicine. Faculty members, fellows, residents and students in the UA Department of Medicine are welcome to use the Divisional Cores.